製品の特長

＊本品は、NPCから中脳型ニューロン前駆体に分化させるための製品です。さらに中脳型ニューロンへと成熟させるにはSTEMdiff™ Midbrain Maturation Kit（ST-100-0041）が必要になります。

STEMdiff™ Midbrain Neuron Differentiation Kitをもちいて、hPSC由来NPCから中脳型ニューロン前駆体を作製できます

• 組成が明確な無血清培地です
• hPSC由来NPCからの、中脳型機能性ニューロンの高効率な作製をサポートします
• TH陽性ニューロン集団 (>15%)を産生し、長期培養で維持できます
• STEMdiff™ SMADi Neural Induction Kitで作製したNPCからの分化に最適化されています
• さまざまなES/iPS細胞株由来のNPCから、中脳型ニューロン前駆体を再現性良く誘導します

データ紹介

STEMdiff™ Midbrain Neuron Differentiation および Maturation Kitで作製した中脳型ニューロン

NPCs generated from H1 hPSCs in mTeSR™1 using the STEMdiff™ SMADi Neural Induction Kit embryoid body (EB) protocol were differentiated and matured to midbrain-type neurons using the STEMdiff™ Midbrain Neuron Differentiation and Maturation Kits. (A) Midbrain-type neurons were formed after hPSC-derived NPCs were cultured with the STEMdiff™ Midbrain Neuron Differentiation Kit for 12 days and STEMdiff™ Midbrain Neuron Maturation Kit for 14 days. The resulting cultures contain a population of (B) class III β-tubulin-positive neurons (magenta), with (D) more than 15% Tyrosine Hydroxylase-positive cells (green). (C) Nuclei are labeled with DAPI (blue).

中脳型ニューロン前駆細胞が発現する特徴的マーカー

NPCs generated from STiPS-F016 hPSCs in mTeSR™1 using the STEMdiff™ SMADi Neural Induction Kit monolayer protocol were differentiated to midbrain-type neural precursors using STEMdiff™ Midbrain Neuron Differentiation Kit for 7 days. (A) Midbrain-type neural precursor cell cultures contain a population of (A) FOXA2-expressing cells (red) and (B) NKX2.1-expressing cells (green). Merge image in (E) showing the bottom half of the same FOV includes DAPI-labeled nuclei (blue) and shows non-overlapping marker expression. The resulting neural precursor cultures also express (C) OTX2 (red) and are negative for (D) central nervous system NPC marker PAX6 (green). Merge image in (F) showing the bottom half of the same FOV includes nuclei that are labeled with DAPI (blue).

中脳型ニューロンが発現するドーパミントランスポーター（Dopamine Transporters; DAT）

NPCs generated from H9 hPSCs in mTeSR™1 using the STEMdiff™ SMADi Neural Induction Kit monolayer protocol were differentiated and matured to midbrain-type neurons using the STEMdiff™ Midbrain Neuron Differentiation and Maturation Kits. (A) Midbrain-type neurons were formed after NPCs were cultured with the STEMdiff™ Midbrain Neuron Differentiation Kit for 12 days and STEMdiff™ Midbrain Neuron Maturation Kit for 14 days. The resulting cultures contain a population of (B) class III β-tubulin-positive neurons (magenta), which (C) express DAT in blue, and (E) Tyrosine Hydroxylase-positive cells (green). (D) Nuclei are labeled with DAPI (white).