STEMCELL Technologies STEMdiff STEMdiff Neural Progenitor Medium
- 研究用
STEMdiff™ Neural Progenitor Medium（ST-05833）は、ヒトES/iPS細胞（hPSC）からSTEMdiff™ Neural Induction Medium（ST-05835）で誘導した神経前駆細胞（NPC）を増殖する無血清培地です。
本品で培養したNPCは継代1回あたり3〜5倍に拡大可能で、自発的な神経分化を最小に抑えながら少なくとも10回の継代培養ができます。
2018/05/14 12:00の製品情報
本製品は研究目的にのみ使用し、人や動物の医療用・臨床診断用・食品用としては使用しないようにご注意ください。
製品の特長
STEMdiff™ Neural Progenitor Mediumで、hPSC由来NPCを維持及び拡大培養
- 無血清の明確な組成
- STEMdiff™ Neural Induction Mediumで作製したNPCの増殖をサポート
- 複数回の継代にわたってNPCが効率よく増殖できるよう最適化済み
- NPCの分化多能性を維持しつつ、自発的な神経分化を最小限に抑制
- 便利で使用しやすいフォーマットとプロトコル
データ紹介
NPCの形態とマーカー発現
Figure 1. Morphology and Marker Expression of Neural Progenitor Cells Cultured in STEMdiff™ Neural Progenitor Medium
(A) Typical NPC morphology is observed in cultures (shown at day 6 of passage 1). (B-D) NPCs maintained in STEMdiff™ Neural Progenitor Medium express the CNS-type NPC markers PAX6 (B, D, red), SOX1 (C, red) and NESTIN (C, green), but not the neural crest marker SOX10 (D, green, single channel shown in inset). B-D were taken at the same magnification.
NPCの増殖
Figure 2. Expansion of Neural Progenitor Cells in STEMdiff™ Neural Progenitor Medium
NPCs cultured in STEMdiff™ Neural Progenitor Medium can be expanded to generate a large number of cells. Three- to five-fold expansion can be achieved upon each passage. NPCs were derived using STEMdiff™ Neural Induction Medium and passaged once a week on average. n = 6.
自発的な神経分化の抑制
Figure 3. Neural Progenitor Cells Cultured in STEMdiff™ Neural Progenitor Medium Show Minimal Spontaneous Neuronal Differentiation
Passages 1 (A) and 3 (B) of a representative NPC culture maintained in STEMdiff™ Neural Progenitor Medium. Cells were immunolabeled with SOX1 (red) to identify NPCs, and class III β-tubulin (green) to identify neurons. Spontaneous neuronal differentiation is low in NPC cultures maintained in STEMdiff™ Neural Progenitor Medium. A and B were taken at the same magnification.
増殖したNPCの、ニューロンとアストロサイトへの分化
Figure 4. Neural Progenitor Cells Maintained in STEMdiff™ Neural Progenitor Medium can Differentiate into Neurons and Astrocytes
When directed according to published protocols, NPCs can differentiate into neurons (A, class III β-tubulin shown in red) and astrocytes (B, GFAP shown in red). Nuclei are counterstained with DAPI (blue).
